Research Use Only — Not for Human or Veterinary Use.

Semaglutide reconstitution calculator

Work out exactly how many units of Semaglutide to draw on an insulin syringe. Enter your vial size and how much bacteriostatic water you added — the maths happens as you type. Free, no account needed.

Peptide in vial
Bacteriostatic (BAC) water added
mL
Desired dose per injection
Draw to
5.0U
on a U-100 · 1 mL insulin syringe
0 U255075100 U
5mg / mL
0.05mL / injection
40doses / vial

Draw 5 units (0.05 mL) on a U-100 · 1 mL for a 0.25 mg dose. Each vial gives about 40 doses.

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How to use this Semaglutide calculator

  1. Enter the vial size printed on your labelSemaglutide is commonly sold as 5 mg, 10 mg, 20 mg.
  2. Enter the bacteriostatic water you added, in mL.
  3. Enter your dose, in mg.
  4. Pick your syringe — the units, fill bar and warnings all adjust to it.

Worked example

A 10 mg Semaglutide vial reconstituted with 2 mL of bacteriostatic water gives a concentration of 5 mg/mL. A 0.25 mg dose is 0.05 mL — which is 5 units on a U-100 insulin syringe, and about 40 doses per vial.

The calculator above is already loaded with these numbers. Change any field to match your own vial.

Typical range (research)
Research literature commonly references escalating weekly amounts in the ~0.25–2.4 mg/week range
Half-life
~7 days
Route
Subcutaneous (research)
Common vial sizes
5 mg · 10 mg · 20 mg
Storage
Store lyophilized powder refrigerated (2–8°C) and protected from light; once reconstituted, keep refrigerated and use within the timeframe supported by stability data.

Common questions

How many units is 0.25 mg of semaglutide?

It depends entirely on your concentration — 'units' measure volume, not peptide. On a 10 mg vial reconstituted with 2 mL (5 mg/mL), 0.25 mg is 0.05 mL, which reads as 5 units on a U-100 insulin syringe. Change the water and the unit count changes even though the dose does not. This is the single most common source of dosing error, and it's exactly what this calculator removes.

Does adding more bacteriostatic water make the dose weaker?

No. It dilutes the concentration, so you draw a larger volume for the same amount of peptide. The milligrams you receive are unchanged — only the number on the syringe moves.

Why do research protocols escalate the amount over time?

Published trials commonly start low and step up over several weeks. Literature attributes this to tolerability, particularly gastrointestinal effects. This is a description of trial design, not a recommendation.

Regulatory note: Prescription drug in Canada and the US; approved for type 2 diabetes and chronic weight management under brand names. Research-grade material is not for human use.

Dose figures are ranges commonly referenced in research literature — not recommendations or medical advice. Research Use Only — Not for Human or Veterinary Use. Always verify any calculation independently. Consult a licensed physician.